Title : the Affects of Temperature on Enzymes Activity

Title the affects of temperature on enzymes activity 1. The Problem measuring rod and recording the focusion of carbohyd grade by enzyme amylase 2. Independent variable water vat temperature 3. certified variable the date taken for the carbohydrates to get digested by amylases 4. Controls the amounts of the carbohydrates and the enzymes in the answer 5. Quantitative measurement Time Temperature Enzymes concentration Substrate concentration Starches Indicators Catalysts In minutes Cloven /Celeste Per millimetre cube Per millimetre cube Per empty No catalyst 1.Materials 6 water bathes. analyze thermionic valves, tryout thermionic vacuum tube rack , amylase enzymes , carbohydrate form ( flower or ,potato), thermometers , indicator , continue measure ,protection (lab coat, goggles ) 2. Procedure * prepare 6 raise tubes with 10 cm of carbohydrate milk and misuse 5 cm of amylase enzymes in a cringe and add to test tube too indicator should be add and there is no catalyst in this experiments * Place the test tubes on the water baths Water baths should be with fixed temperatures from 10 ,20,30,40,50,60,70 * run the stop clock as soon as the test tube in the water bath * Keep an eye on the test tube till colour change is observed and the take the test tube out and tack time measurements * in any case every temperature measurements should be done individually that the other tests to eliminate error (no one bottomland be in 6 places at the same time ) * shake a table and record the time taken to the enzymes to work and digest the substrate at each temperature individually *Draw a chart Make your conclusion based on the ensues Hypothesis. the affect of temperatures on rate of typical enzyme controlled reaction up to about 40c the rate increases smoothly, also a 10 degree increase of the temperature accompanied by dear doubling in of the rate of the reaction this is the normal temperature rule of chemical substance reactions in general, ab ove this temperature the rate starts to fall off then spurn rapidly . above 60 degree the rate ceases or stop completely. . Explanation increase of reaction why? Increases in the temperature of a outline results from increases in the kinetic energy of the system. This has several effects on the rates of reactions, 1-the increase in temperature will increase the activation energy of a molecules there for it has more potential to react and change status Also the temperature will make the molecules move faster and as a result more collision will be possible per/minute . Enzymes activity stops why? Altogether and that is because of the heat changes the shape of the enzyme molecules preventing them from working ,this is called (denaturation ) in fact enzymes will denature and any temperature that is why they cannot be used over and over again ,however the higher the temperature the less time it takes for the denaturation to occurs . 5. danger assessmentHazard Risk How to avoid Wha t happens in case of accident Risk probability to occurs Heat from hot water bath Burning Handel with address strain medical ease Big risk Enzymes Biological hazards (irritant Handel with sort out equipment Wash under water tape Medium Glass equipment trauma or a glass cute Try to handel with care Seek medical help Big Thermometer In case it breaks contain hydrargyrum Handle with care Dont move it from the water bath unless by the lab specialist Seek eargent medical help in case of contact with mercury Big Electrical wire from water baths Electrocuted or starting a fire But it out of the charge not in contact with water Seek medical help Small Source Reference Was it useful Used for Advanced biota Michael Kent, 2004. Advanced Biology. Edition. Oxford University Press yes Background information Biology Principles and Processes M B V Roberts, 1993. Biology Principles and Processes Pb. Edition. Thomas Nelson. Yes Methods Web inwardness of temper ature on enzyme activity. 2012. Effect of temperature on enzyme activity. ONLINE Available at http//academic. brooklyn. cuny. edu/biology/bio4fv/page/enz_act. htm. Accessed 13 November 2012. Yes